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A total of Klebsiella strains collected from previous surveillance studies were randomly selected for this study, in order to represent distinct Brazilian geographic regions. Journal List Antimicrob Jcn Chemother v.
Antimicrobial susceptibility testing was performed by the broth microdilution method, according to CLSI guidelines 45. To determine whether this plasmid was identical to p, the first plasmid identified as carrying bla BKC-1we performed whole-plasmid sequencing of the plasmid recovered from KpnJ.
In conclusion, prudent use of antimicrobials and strict adherence to infection control measures seem to be the only hcm tools for preventing the acquisition and spread of new carbapenemase-encoding genes.
Only 2 BKCproducing K. Antimicrobial susceptibility profiles of BKCproducing K.
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The principal objective of this study was to evaluate the frequency of bla BKC-1 by testing a collection of Klebsiella isolates. Support Center Support Center. Prepublished online May To date, inactivation of mgrB has been the main mechanism of polymyxin resistance observed in K. Author information Article notes Copyright and License information Disclaimer.
J Antimicrob Chemother Martinsa Adriana G. This article has been cited by other articles in PMC. S, sensitive; R, resistant.
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The other authors have no conflicts to declare. Multilocus sequence typing of Klebsiella pneumoniae nosocomial isolates. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; approved standard— 10th ed CLSI document MA Only 2 of Klebsiella isolates 0.
Antimicrob Agents Chemother J Clin Microbiol Sampaiob, c and Ana C. Clinical and Laboratory Standards Institute.
Plasmid extraction of K. Transformant cells were selected on LB agar plates supplemented with 0. The Kpn and KpnJ strains showed resistance to all beta-lactams and fluoroquinolones. National Center for Biotechnology InformationU.
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The Klebsiella strains were isolated from different clinical specimens obtained between and Fig. Since BKC-1 is a weak carbapenemase and other mechanisms could be associated with carbapenemase production, we selected Klebsiella strains independent of the resistance phenotype.
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Diagn Microbiol Infect Dis During the experiments, it was observed that bla BKC-1 was easily lost in the absence of selective pressure. Initially, we performed transformation by electroporation into Escherichia coli Top Kpn showed susceptibility to tigecycline MIC, 0. The Kpn and KpnJ strains showed different plasmid profiles.